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Clonogrouping, a Rapid Multiplex PCR Method for Identification of Major Clones of Listeria monocytogenes.

ABSTRACT: Three multiplex PCR assays were developed to identify the 11 most common Listeria monocytogenes clones in clinical and food samples; 270 (95.7%) of 282 strains of serogroups IVb, IIb, IIa, and IIc were identified accurately. This novel tool is a rapid and efficient alternative to multilocus sequence typing for identification of L. monocytogenes clones.

SUBMITTER: Chenal-Francisque V

PROVIDER: S-EPMC4572523 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature

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Clonogrouping, a Rapid Multiplex PCR Method for Identification of Major Clones of Listeria monocytogenes.

Chenal-Francisque Viviane V Maury Mylène M MM Lavina Morgane M Touchon Marie M Leclercq Alexandre A Lecuit Marc M Brisse Sylvain S

Journal of clinical microbiology 20150729 10

Three multiplex PCR assays were developed to identify the 11 most common Listeria monocytogenes clones in clinical and food samples; 270 (95.7%) of 282 strains of serogroups IVb, IIb, IIa, and IIc were identified accurately. This novel tool is a rapid and efficient alternative to multilocus sequence typing for identification of L. monocytogenes clones. ...[more]

PMID: 26224848

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