MicroSAGE is highly representative and reproducible but reveals major differences in gene expression among samples obtained from similar tissues.
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ABSTRACT: Background
Serial analysis of gene expression using small amounts of starting material (microSAGE) has not yet been conclusively shown to be representative, reproducible or accurate.Results
We show that microSAGE is highly representative, reproducible and accurate, but that pronounced differences in gene expression are seen between tissue samples taken from different individuals.Conclusions
MicroSAGE is a reliable method of comprehensively profiling differences in gene expression among samples, but care should be taken in generalizing results obtained from libraries constructed from tissue obtained from different individuals and/or processed or stored differently.
SUBMITTER: Blackshaw S
PROVIDER: S-EPMC153457 | biostudies-literature | 2003
REPOSITORIES: biostudies-literature
Publications
MicroSAGE is highly representative and reproducible but reveals major differences in gene expression among samples obtained from similar tissues.
Blackshaw Seth S Kuo Winston P WP Park Peter J PJ Tsujikawa Motokazu M Gunnersen Jenny M JM Scott Hamish S HS Boon Wee-Ming WM Tan Seong-Seng SS Cepko Constance L CL
Genome biology 20030218 3
<h4>Background</h4>Serial analysis of gene expression using small amounts of starting material (microSAGE) has not yet been conclusively shown to be representative, reproducible or accurate.<h4>Results</h4>We show that microSAGE is highly representative, reproducible and accurate, but that pronounced differences in gene expression are seen between tissue samples taken from different individuals.<h4>Conclusions</h4>MicroSAGE is a reliable method of comprehensively profiling differences in gene ex ...[more]
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