MicroSAGE is highly representative and reproducible but reveals major differences in gene expression among samples obtained from similar tissues.
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ABSTRACT:
SUBMITTER: Blackshaw S
PROVIDER: S-EPMC153457 | biostudies-literature | 2003
REPOSITORIES: biostudies-literature
Publications
MicroSAGE is highly representative and reproducible but reveals major differences in gene expression among samples obtained from similar tissues.
Blackshaw Seth S Kuo Winston P WP Park Peter J PJ Tsujikawa Motokazu M Gunnersen Jenny M JM Scott Hamish S HS Boon Wee-Ming WM Tan Seong-Seng SS Cepko Constance L CL
Genome biology 20030218 3
<h4>Background</h4>Serial analysis of gene expression using small amounts of starting material (microSAGE) has not yet been conclusively shown to be representative, reproducible or accurate.<h4>Results</h4>We show that microSAGE is highly representative, reproducible and accurate, but that pronounced differences in gene expression are seen between tissue samples taken from different individuals.<h4>Conclusions</h4>MicroSAGE is a reliable method of comprehensively profiling differences in gene ex ...[more]
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