Unknown

Dataset Information

0

Expression of herpes simplex virus ICP0 inhibits the induction of interferon-stimulated genes by viral infection.


ABSTRACT: The herpes simplex virus type 1 (HSV-1) mutant d109 does not express any of the immediate-early (IE) proteins and persists in cells for a prolonged length of time. As has been shown by Nicholl et al. (J. Gen. Virol. 81:2215-2218, 2000) and Mossman et al. (J. Virol. 75:750-758, 2001) using other mutants defective for IE gene expression, infection with d109 induced the expression of a number of interferon-stimulated genes. Induction of these genes was significantly greater at multiplicities of infection (MOI) of 10 PFU/cell or greater, and the resulting antiviral effect was only seen at MOIs greater than 10 PFU/cell. Using mutants defective for sets of IE genes established that the lack of ICP0 expression was necessary for high levels of interferon-stimulated gene expression in HEL cells. The induction of interferon-stimulated genes by d109 could also be inhibited by infection with an E1-:E3-:E4- adenovirus expressing levels of ICP0 that are comparable to those expressed within the first hour of wild-type virus infection. Lastly, the addition of the proteasome inhibitor MG132 to cells infected with a mutant that expresses ICP0, d106, also resulted in the induction of interferon-stimulated genes. Thus, ICP0 may function through the proteasome very early in HSV infection to inhibit a cellular antiviral response induced by the virion.

SUBMITTER: Eidson KM 

PROVIDER: S-EPMC153810 | biostudies-literature | 2002 Mar

REPOSITORIES: biostudies-literature

altmetric image

Publications

Expression of herpes simplex virus ICP0 inhibits the induction of interferon-stimulated genes by viral infection.

Eidson Kasey M KM   Hobbs William E WE   Manning Brian J BJ   Carlson Paul P   DeLuca Neal A NA  

Journal of virology 20020301 5


The herpes simplex virus type 1 (HSV-1) mutant d109 does not express any of the immediate-early (IE) proteins and persists in cells for a prolonged length of time. As has been shown by Nicholl et al. (J. Gen. Virol. 81:2215-2218, 2000) and Mossman et al. (J. Virol. 75:750-758, 2001) using other mutants defective for IE gene expression, infection with d109 induced the expression of a number of interferon-stimulated genes. Induction of these genes was significantly greater at multiplicities of inf  ...[more]

Similar Datasets

| S-EPMC2593313 | biostudies-literature
| S-EPMC7747705 | biostudies-literature
| S-EPMC2809113 | biostudies-literature
| S-EPMC3082435 | biostudies-literature
| S-EPMC2882321 | biostudies-literature
| S-EPMC4645643 | biostudies-literature
| S-EPMC3447814 | biostudies-literature
| S-EPMC4077717 | biostudies-literature
| S-EPMC3067832 | biostudies-literature
| S-EPMC2849514 | biostudies-literature