Constitutive activation of two-component response regulators: characterization of VirG activation in Agrobacterium tumefaciens.

ABSTRACT: Response regulators are the ultimate modulators in two-component signal transduction pathways. The N-terminal receiver domains generally accept phosphates from cognate histidine kinases to control output. VirG for example, the response regulator of the VirA/VirG two-component system in Agrobacterium tumefaciens, mediates the expression of virulence genes in response to plant host signals. Response regulators have a highly conserved structure and share a similar conformational activation upon phosphorylation, yet the sequence and structural features that determine or perturb the cooperative activation events are ill defined. Here we use VirG and the unique features of the Agrobacterium system to extend our understanding of the response regulator activation. Two previously isolated constitut

SUBMITTER: Gao R 

PROVIDER: S-EPMC1539974 | biostudies-literature | 2006 Jul

REPOSITORIES: biostudies-literature