Ontology highlight
ABSTRACT:
SUBMITTER: Chen YS
PROVIDER: S-EPMC154968 | biostudies-literature | 2003 May
REPOSITORIES: biostudies-literature
Chen Yao-Shen YS Shiuan David D Chen Ssu-Ching SC Chye Soi-Moi SM Chen Ya-Lei YL
Clinical and diagnostic laboratory immunology 20030501 3
Current serological tests for melioidosis, using impure or uncharacterized cell antigens from Burkholderia pseudomallei, have problems in detection sensitivity and specificity. Therefore, we designed and expressed the recombinant flagellin (truncated at both the N- and C-terminal ends), and used the antigen to develop an indirect enzyme-linked immunosorbent assay (ELISA) to diagnose melioidosis. Comparison of the immunoreactivities of the full-length and truncated flagellins reveals that the tru ...[more]