Ontology highlight
ABSTRACT:
SUBMITTER: Sandig V
PROVIDER: S-EPMC15501 | biostudies-literature | 2000 Feb
REPOSITORIES: biostudies-literature
Sandig V V Youil R R Bett A J AJ Franlin L L LL Oshima M M Maione D D Wang F F Metzker M L ML Savino R R Caskey C T CT
Proceedings of the National Academy of Sciences of the United States of America 20000201 3
Helper-dependent (HD) adenoviral vectors devoid of all viral coding sequences provide for safe and highly efficient gene transfer with long-lasting transgene expression. High titer stocks of HD vectors can be generated by using the cre-recombinase system. However, we have encountered difficulties with this system, including rearranged HD vectors and variable efficiency of HD vector rescue. These problems represent a major hindrance, particularly with regard to large-scale production. To overcome ...[more]