Ontology highlight
ABSTRACT:
SUBMITTER: Taylor AM
PROVIDER: S-EPMC1558906 | biostudies-literature | 2005 Aug
REPOSITORIES: biostudies-literature
Taylor Anne M AM Blurton-Jones Mathew M Rhee Seog Woo SW Cribbs David H DH Cotman Carl W CW Jeon Noo Li NL
Nature methods 20050801 8
Investigation of axonal biology in the central nervous system (CNS) is hindered by a lack of an appropriate in vitro method to probe axons independently from cell bodies. Here we describe a microfluidic culture platform that polarizes the growth of CNS axons into a fluidically isolated environment without the use of targeting neurotrophins. In addition to its compatibility with live cell imaging, the platform can be used to (i) isolate CNS axons without somata or dendrites, facilitating biochemi ...[more]