Unknown

Dataset Information

0

ITK and IL-15 support two distinct subsets of CD8+ T cells.


ABSTRACT: CD8(+) T cells are commonly divided into naïve CD44(lo)CD122(lo) and "memory phenotype" CD44(hi)CD122(hi) cells. Here we show data suggesting that these two cell populations represent independent CD8(+) T cell subsets. Whereas IL-15(-/-) mice lack CD44(hi)CD122(hi) CD8(+) T cells, mice deficient in the kinase ITK lack CD44(lo)CD122(lo) cells among CD8(+) T cells. The same defects were observed during thymus development. CD44(hi)CD122(hi) cells were found among double-positive thymocytes and increased in frequency during CD8 development in wild-type mice. At the mature stage, IL-15(-/-) mice harbored virtually no CD44(hi)CD122(hi) CD8(+) thymocytes. In contrast, ITK(-/-) mice lacked CD44(lo)CD122(lo) CD8(+) cells at this stage. We generated mice with genetic deletions in both IL-15 and ITK and observed a severe reduction of all CD8(+) T cells. The two CD44(lo)CD122(lo) and CD44(hi)CD122(hi) CD8(+) T cell subsets differed in the periphery in that natural killer (NK) receptor expression was found only on CD44(hi)CD122(hi) CD8(+) T cells. This expression was paralleled by their ability to respond to both T cell receptor and NK receptor engagements. In contrast, CD44(lo)CD122(lo) CD8(+) T cells mounted stronger responses to T cell receptor stimulation but failed to recognize NK receptor ligands. Thus, whereas ITK-dependent CD44(lo)CD122(lo) CD8(+) T cells appear to represent conventional CD8(+) T cells, IL-15-dependent CD44(hi)CD122(hi) CD8(+) T cells may have functions in both adaptive and innate immunity.

SUBMITTER: Dubois S 

PROVIDER: S-EPMC1567699 | biostudies-literature | 2006 Aug

REPOSITORIES: biostudies-literature

altmetric image

Publications

ITK and IL-15 support two distinct subsets of CD8+ T cells.

Dubois Sigrid S   Waldmann Thomas A TA   Müller Jürgen R JR  

Proceedings of the National Academy of Sciences of the United States of America 20060731 32


CD8(+) T cells are commonly divided into naïve CD44(lo)CD122(lo) and "memory phenotype" CD44(hi)CD122(hi) cells. Here we show data suggesting that these two cell populations represent independent CD8(+) T cell subsets. Whereas IL-15(-/-) mice lack CD44(hi)CD122(hi) CD8(+) T cells, mice deficient in the kinase ITK lack CD44(lo)CD122(lo) cells among CD8(+) T cells. The same defects were observed during thymus development. CD44(hi)CD122(hi) cells were found among double-positive thymocytes and incr  ...[more]

Similar Datasets

| S-EPMC2572798 | biostudies-other
| S-EPMC2950111 | biostudies-literature
| S-EPMC524066 | biostudies-literature
| S-EPMC4114307 | biostudies-literature
| S-EPMC1766429 | biostudies-literature
| S-EPMC3110618 | biostudies-other
| S-EPMC2689280 | biostudies-literature
| S-EPMC7720079 | biostudies-literature
| S-EPMC4922693 | biostudies-literature
| S-EPMC5145194 | biostudies-literature