Ontology highlight
ABSTRACT:
SUBMITTER: Dietz H
PROVIDER: S-EPMC1568916 | biostudies-literature | 2006 Aug
REPOSITORIES: biostudies-literature
Dietz Hendrik H Berkemeier Felix F Bertz Morten M Rief Matthias M
Proceedings of the National Academy of Sciences of the United States of America 20060814 34
Single-molecule methods have given experimental access to the mechanical properties of single protein molecules. So far, access has been limited to mostly one spatial direction of force application. Here, we report single-molecule experiments that explore the mechanical properties of a folded protein structure in precisely controlled directions by applying force to selected amino acid pairs. We investigated the deformation response of GFP in five selected directions. We found fracture forces wid ...[more]