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Electrophoretic Deformation of Individual Transfer RNA Molecules Reveals Their Identity.

ABSTRACT: It has been hypothesized that the ribosome gains additional fidelity during protein translation by probing structural differences in tRNA species. We measure the translocation kinetics of different tRNA species through ?3 nm diameter synthetic nanopores. Each tRNA species varies in the time scale with which it is deformed from equilibrium, as in the translocation step of protein translation. Using machine-learning algorithms, we can differentiate among five tRNA species, analyze the ratios of tRNA binary mixtures, and distinguish tRNA isoacceptors.

SUBMITTER: Henley RY

PROVIDER: S-EPMC4890568 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature

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Electrophoretic Deformation of Individual Transfer RNA Molecules Reveals Their Identity.

Henley Robert Y RY Ashcroft Brian Alan BA Farrell Ian I Cooperman Barry S BS Lindsay Stuart M SM Wanunu Meni M

Nano letters 20151202 1

It has been hypothesized that the ribosome gains additional fidelity during protein translation by probing structural differences in tRNA species. We measure the translocation kinetics of different tRNA species through ∼3 nm diameter synthetic nanopores. Each tRNA species varies in the time scale with which it is deformed from equilibrium, as in the translocation step of protein translation. Using machine-learning algorithms, we can differentiate among five tRNA species, analyze the ratios of tR ...[more]

PMID: 26609994

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