Ontology highlight
ABSTRACT:
SUBMITTER: Dreier J
PROVIDER: S-EPMC1594635 | biostudies-literature | 2006 Aug
REPOSITORIES: biostudies-literature
Dreier Jens J Störmer Melanie M Mäde Dietrich D Burkhardt Sabine S Kleesiek Knut K
Journal of clinical microbiology 20060801 8
We have developed a one-tube reverse transcription (RT)-PCR method using the real-time TaqMan PCR system for the detection of norovirus genogroup I (NV GGI). By introduction of a novel probe based on locked nucleic acid technology, we enhanced the sensitivity of the assay compared to those of conventional TaqMan probes. The sensitivity of the NV GGI RT-PCR was determined by probit analysis with defined RNA standards and quantified norovirus isolates to 711 copies/ml (95% detection limit). In ord ...[more]