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Development of a reverse transcription-quantitative PCR assay for detection of salivirus/klassevirus.

ABSTRACT: A broadly reactive and highly sensitive reverse transcription-quantitative PCR assay to detect salivirus/klassevirus was developed. By means of the developed assay, salivirus/klassevirus was detected in 13 (93%) raw sewage, 4 (29%) secondary-treated sewage, and 9 (16%) river water samples, with a maximum concentration of 9.7 × 10(6) copies/liter.

SUBMITTER: Haramoto E 

PROVIDER: S-EPMC3648030 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature

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Development of a reverse transcription-quantitative PCR assay for detection of salivirus/klassevirus.

Haramoto Eiji E   Kitajima Masaaki M   Otagiri Mikie M  

Applied and environmental microbiology 20130329 11

A broadly reactive and highly sensitive reverse transcription-quantitative PCR assay to detect salivirus/klassevirus was developed. By means of the developed assay, salivirus/klassevirus was detected in 13 (93%) raw sewage, 4 (29%) secondary-treated sewage, and 9 (16%) river water samples, with a maximum concentration of 9.7 × 10(6) copies/liter. ...[more]

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