Ontology highlight
ABSTRACT:
SUBMITTER: Huang A
PROVIDER: S-EPMC1594736 | biostudies-literature | 2006 Sep
REPOSITORIES: biostudies-literature
Journal of clinical microbiology 20060901 9
Here we report the development of an oligonucleotide microarray method that can identify fungal pathogens in a single reaction. Specific oligonucleotide probes targeted to internal transcribed spacer 2 were designed and synthesized. Fungal DNA was amplified by universal primers, and the PCR product was hybridized with the oligonucleotide microarray. A series of specific hybridization profiles corresponding to species were obtained. The 122 strains of fungal pathogens, including standard and clin ...[more]