Ontology highlight
ABSTRACT:
SUBMITTER: Ishibashi K
PROVIDER: S-EPMC1595443 | biostudies-literature | 2006 Oct
REPOSITORIES: biostudies-literature
Ishibashi Kazuma K Suzuki Keiichiro K Ando Yoshinori Y Takakura Chihiro C Inoue Hirokazu H
Proceedings of the National Academy of Sciences of the United States of America 20060926 40
Homologous integration of a foreign DNA segment into a chromosomal target sequence enables precise disruption or replacement of genes of interest and provides an effective means to analyze gene function. However, integration after transformation is predominantly nonhomologous in most species other than yeast. Here, we show that homologous integration in the filamentous fungus Neurospora requires the homologous-recombination proteins MEI-3 (yeast Rad51 homolog) and MUS-25 (yeast Rad54 homolog), w ...[more]