Ontology highlight
ABSTRACT:
SUBMITTER: Saito S
PROVIDER: S-EPMC5508229 | biostudies-literature | 2017 Jul
REPOSITORIES: biostudies-literature
Saito Shinta S Maeda Ryo R Adachi Noritaka N
Nature communications 20170711
Homologous recombination-mediated gene targeting has greatly contributed to genetic analysis in a wide range of species, but is highly inefficient in human cells because of overwhelmingly frequent random integration events, whose molecular mechanism remains elusive. Here we show that DNA polymerase θ, despite its minor role in chromosomal DNA repair, substantially contributes to random integration, and that cells lacking both DNA polymerase θ and DNA ligase IV, which is essential for non-homolog ...[more]