Precise centromere mapping using a combination of repeat junction markers and chromatin immunoprecipitation-polymerase chain reaction.
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ABSTRACT:
SUBMITTER: Luce AC
PROVIDER: S-EPMC1602074 | biostudies-literature | 2006 Oct
REPOSITORIES: biostudies-literature
Publications
Precise centromere mapping using a combination of repeat junction markers and chromatin immunoprecipitation-polymerase chain reaction.
Luce Amy C AC Sharma Anupma A Mollere Oliver S B OS Wolfgruber Thomas K TK Nagaki Kiyotaka K Jiang Jiming J Presting Gernot G GG Dawe R Kelly RK
Genetics 20060901 2
Centromeres are difficult to map even in species where genetic resolution is excellent. Here we show that junctions between repeats provide reliable single-copy markers for recombinant inbred mapping within centromeres and pericentromeric heterochromatin. Repeat junction mapping was combined with anti-CENH3-mediated ChIP to provide a definitive map position for maize centromere 8. ...[more]
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