Unknown

Dataset Information

0

Combination primer polymerase chain reaction for multi-site mutagenesis of close proximity sites.


ABSTRACT: We describe a rapid and efficient polymerase chain reaction procedure for multi-site-directed mutagenesis for cases in which the sites to be mutated are in close proximity. The combination primer polymer chain reaction method is based on a multi-site directed mutagenesis protocol together with a splicing by overlapping extension polymerase chain reaction protocol. several different combinations of multiple mutations were successfully performed with this method and are reported in this study.

SUBMITTER: Jensen PH 

PROVIDER: S-EPMC2291755 | biostudies-literature | 2005 Dec

REPOSITORIES: biostudies-literature

altmetric image

Publications

Combination primer polymerase chain reaction for multi-site mutagenesis of close proximity sites.

Jensen Pia Hønnerup PH   Weilguny Dietmar D  

Journal of biomolecular techniques : JBT 20051201 4


We describe a rapid and efficient polymerase chain reaction procedure for multi-site-directed mutagenesis for cases in which the sites to be mutated are in close proximity. The combination primer polymer chain reaction method is based on a multi-site directed mutagenesis protocol together with a splicing by overlapping extension polymerase chain reaction protocol. several different combinations of multiple mutations were successfully performed with this method and are reported in this study. ...[more]

Similar Datasets

| S-EPMC4440812 | biostudies-literature
| S-EPMC7383071 | biostudies-literature
| S-EPMC3412702 | biostudies-literature
| S-EPMC5327792 | biostudies-literature
| S-EPMC5705716 | biostudies-literature
| S-EPMC102640 | biostudies-literature
| S-EPMC6573857 | biostudies-literature
| S-EPMC5643884 | biostudies-literature
| S-EPMC4490387 | biostudies-other
| 2756739 | ecrin-mdr-crc