Ontology highlight
ABSTRACT:
SUBMITTER: Jensen PH
PROVIDER: S-EPMC2291755 | biostudies-literature | 2005 Dec
REPOSITORIES: biostudies-literature
Jensen Pia Hønnerup PH Weilguny Dietmar D
Journal of biomolecular techniques : JBT 20051201 4
We describe a rapid and efficient polymerase chain reaction procedure for multi-site-directed mutagenesis for cases in which the sites to be mutated are in close proximity. The combination primer polymer chain reaction method is based on a multi-site directed mutagenesis protocol together with a splicing by overlapping extension polymerase chain reaction protocol. several different combinations of multiple mutations were successfully performed with this method and are reported in this study. ...[more]