Ontology highlight
ABSTRACT:
SUBMITTER: Ceccarelli M
PROVIDER: S-EPMC1614504 | biostudies-literature | 2006 Nov
REPOSITORIES: biostudies-literature
Ceccarelli Matteo M Ruggerone Paolo P Anedda Roberto R Fais Antonella A Era Benedetta B Sollaino Maria Carla MC Corda Marcella M Casu Mariano M
Biophysical journal 20060714 9
Our study examines the functional and structural effects of amino acid substitution in the distal side of beta-chains of human Hb Duarte (alpha(2)beta(2)(62Ala-->Pro)). We have compared the functional properties of the purified Hb Duarte with those of HbA, and through proton NMR and molecular dynamics simulations we have investigated their tertiary and quaternary structures. The variant exhibits an increased oxygen affinity with a normal Hill coefficient and Bohr effect. The abnormal function of ...[more]