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Role of HuR in skeletal myogenesis through coordinate regulation of muscle differentiation genes.


ABSTRACT: In this report, we investigate the role of the RNA-binding protein HuR during skeletal myogenesis. At the onset of myogenesis in differentiating C2C12 myocytes and in vivo in regenerating mouse muscle, HuR cytoplasmic abundance increased dramatically, returning to a predominantly nuclear presence upon completion of myogenesis. mRNAs encoding key regulators of myogenesis-specific transcription (myogenin and MyoD) and cell cycle withdrawal (p21), bearing AU-rich regions, were found to be targets of HuR in a differentiation-dependent manner. Accordingly, mRNA half-lives were highest during differentiation, declining when differentiation was completed. Importantly, HuR-overexpressing C2C12 cells displayed increased target mRNA expression and half-life and underwent precocious differentiation. Our findings underscore a critical function for HuR during skeletal myogenesis linked to HuR's coordinate regulation of muscle differentiation genes.

SUBMITTER: Figueroa A 

PROVIDER: S-EPMC162217 | biostudies-literature | 2003 Jul

REPOSITORIES: biostudies-literature

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Role of HuR in skeletal myogenesis through coordinate regulation of muscle differentiation genes.

Figueroa Angélica A   Cuadrado Ana A   Fan Jinshui J   Atasoy Ulus U   Muscat George E GE   Muñoz-Canoves Pura P   Gorospe Myriam M   Muñoz Alberto A  

Molecular and cellular biology 20030701 14


In this report, we investigate the role of the RNA-binding protein HuR during skeletal myogenesis. At the onset of myogenesis in differentiating C2C12 myocytes and in vivo in regenerating mouse muscle, HuR cytoplasmic abundance increased dramatically, returning to a predominantly nuclear presence upon completion of myogenesis. mRNAs encoding key regulators of myogenesis-specific transcription (myogenin and MyoD) and cell cycle withdrawal (p21), bearing AU-rich regions, were found to be targets o  ...[more]

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