Ontology highlight
ABSTRACT:
SUBMITTER: Larsen C
PROVIDER: S-EPMC1635159 | biostudies-literature | 2006 Nov
REPOSITORIES: biostudies-literature
Larsen Camilla C Franch-Marro Xavier X Hartenstein Volker V Alexandre Cyrille C Vincent Jean-Paul JP
Proceedings of the National Academy of Sciences of the United States of America 20061108 47
Transposable elements have been used in Drosophila to detect gene expression, inactivate gene function, and induce ectopic expression or overexpression. We have combined all of these features in a single construct. A promoterless GAL4 cDNA is expressed when the construct inserts within a transcriptional unit, and GAL4 activates a GFP-encoding gene present in the same transposon. In a primary screen, patterned gene expression is detected as GFP fluorescence in the live progeny of dysgenic males. ...[more]