Ontology highlight
ABSTRACT:
SUBMITTER: Kuwahara M
PROVIDER: S-EPMC1636466 | biostudies-literature | 2006
REPOSITORIES: biostudies-literature
Kuwahara Masayasu M Nagashima Jun-ichi J Hasegawa Masatoshi M Tamura Takehiro T Kitagata Rina R Hanawa Kazuo K Hososhima Shin-ichi S Kasamatsu Toshiyuki T Ozaki Hiroaki H Sawai Hiroaki H
Nucleic acids research 20060929 19
We synthesized C5-modified analogs of 2'-deoxyuridine triphosphate and 2'-deoxycytidine triphosphate and investigated them as substrates for PCRs using Taq, Tth, Vent(exo-), KOD Dash and KOD(exo-) polymerases and pUC 18 plasmid DNA as a template. These assays were performed on two different amplifying regions of pUC18 with different T/C contents that are expected to have relatively high barriers for incorporation of either modified dU or dC. On the basis of 260 different assays (26 modified trip ...[more]