Ontology highlight
ABSTRACT:
SUBMITTER: Percipalle P
PROVIDER: S-EPMC164471 | biostudies-literature | 2003 May
REPOSITORIES: biostudies-literature
Proceedings of the National Academy of Sciences of the United States of America 20030512 11
To determine the function of actin in the cell nucleus, we sought to identify nuclear actin-binding proteins in the dipteran Chironomus tentans using DNase I-affinity chromatography. We identified the RNA-binding protein hrp65 as an actin-binding protein and showed that the C-terminal sequence of the hrp65-2 isoform is able to interact directly with actin in vitro. In vivo crosslinking and coimmunoprecipitation experiments indicated that hrp65 and actin are also associated in the living cell. Mo ...[more]