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Chromosomal elements regulate gene activity and chromatin structure of the human serpin gene cluster at 14q32.1.

ABSTRACT: The human serine protease inhibitor (serpin) gene cluster at 14q32.1 contains a number of genes that are specifically expressed in hepatic cells. Cell-specific enhancers have been identified in several of these genes, but elements involved in locus-wide gene and chromatin control have yet to be defined. To identify regulatory elements in this region, we prepared a series of mutant chromosomal alleles by homologous recombination and transferred the specifically modified human chromosomes to hepatic cells for functional tests. We report that deletion of an 8-kb DNA segment upstream of the human alpha1-antitrypsin gene yields a mutant serpin allele that fails to be activated in hepatic cells. Within this region, a 2.3-kb DNA segment between kb -8.1 and -5.8 contains a previously unrecognized control region that is required not only for serpin gene activation but also for chromatin remodeling of the entire locus.

SUBMITTER: Marsden MD

PROVIDER: S-EPMC164764 | biostudies-literature | 2003 May

REPOSITORIES: biostudies-literature

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Chromosomal elements regulate gene activity and chromatin structure of the human serpin gene cluster at 14q32.1.

Marsden Mark D MD Fournier R E K RE

Molecular and cellular biology 20030501 10

The human serine protease inhibitor (serpin) gene cluster at 14q32.1 contains a number of genes that are specifically expressed in hepatic cells. Cell-specific enhancers have been identified in several of these genes, but elements involved in locus-wide gene and chromatin control have yet to be defined. To identify regulatory elements in this region, we prepared a series of mutant chromosomal alleles by homologous recombination and transferred the specifically modified human chromosomes to hepat ...[more]

PMID: 12724410

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