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RNA-binding protein HuR enhances p53 translation in response to ultraviolet light irradiation.


ABSTRACT: Exposure to short-wavelength UV light (UVC) strongly induces p53 expression. In human RKO colorectal carcinoma cells, this increase was not due to elevated p53 mRNA abundance, cytoplasmic export of p53 mRNA, or UVC-triggered stabilization of the p53 protein. Instead, p53 translation was potently enhanced after UVC irradiation. The 3' UTR of p53 was found to be a target of the RNA-binding protein HuR in a UVC-dependent manner in vitro and in vivo. HuR-overexpressing RKO cells displayed elevated p53 levels, whereas cells expressing reduced HuR showed markedly diminished p53 abundance and p53 translation. Our results demonstrate a role for HuR in binding to the p53 mRNA and enhancing its translation.

SUBMITTER: Mazan-Mamczarz K 

PROVIDER: S-EPMC166233 | biostudies-literature | 2003 Jul

REPOSITORIES: biostudies-literature

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RNA-binding protein HuR enhances p53 translation in response to ultraviolet light irradiation.

Mazan-Mamczarz Krystyna K   Galbán Stefanie S   López de Silanes Isabel I   Martindale Jennifer L JL   Atasoy Ulus U   Keene Jack D JD   Gorospe Myriam M  

Proceedings of the National Academy of Sciences of the United States of America 20030623 14


Exposure to short-wavelength UV light (UVC) strongly induces p53 expression. In human RKO colorectal carcinoma cells, this increase was not due to elevated p53 mRNA abundance, cytoplasmic export of p53 mRNA, or UVC-triggered stabilization of the p53 protein. Instead, p53 translation was potently enhanced after UVC irradiation. The 3' UTR of p53 was found to be a target of the RNA-binding protein HuR in a UVC-dependent manner in vitro and in vivo. HuR-overexpressing RKO cells displayed elevated p  ...[more]

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