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Core transcriptional regulatory circuitry in human hepatocytes.

ABSTRACT: We mapped the transcriptional regulatory circuitry for six master regulators in human hepatocytes using chromatin immunoprecipitation and high-resolution promoter microarrays. The results show that these regulators form a highly interconnected core circuitry, and reveal the local regulatory network motifs created by regulator-gene interactions. Autoregulation was a prominent theme among these regulators. We found that hepatocyte master regulators tend to bind promoter regions combinatorially and that the number of transcription factors bound to a promoter corresponds with observed gene expression. Our studies reveal portions of the core circuitry of human hepatocytes.

SUBMITTER: Odom DT

PROVIDER: S-EPMC1681491 | biostudies-literature | 2006

REPOSITORIES: biostudies-literature

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Core transcriptional regulatory circuitry in human hepatocytes.

Odom Duncan T DT Dowell Robin D RD Jacobsen Elizabeth S ES Nekludova Lena L Rolfe P Alexander PA Danford Timothy W TW Gifford David K DK Fraenkel Ernest E Bell Graeme I GI Young Richard A RA

Molecular systems biology 20060502

We mapped the transcriptional regulatory circuitry for six master regulators in human hepatocytes using chromatin immunoprecipitation and high-resolution promoter microarrays. The results show that these regulators form a highly interconnected core circuitry, and reveal the local regulatory network motifs created by regulator-gene interactions. Autoregulation was a prominent theme among these regulators. We found that hepatocyte master regulators tend to bind promoter regions combinatorially and ...[more]

PMID: 16738562

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