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Molecular method for identification of Rickettsia species in clinical and environmental samples.


ABSTRACT: We present a PCR method targeting the 23S-5S internal transcribed spacer coupled with reverse line blotting that allows Rickettsia species detection and identification in a single step. The method is highly sensitive and specific in identifying Rickettsia species from both patient and environmental samples. The generic approach used allowed us to identify new pathogens.

SUBMITTER: Jado I 

PROVIDER: S-EPMC1698399 | biostudies-literature | 2006 Dec

REPOSITORIES: biostudies-literature

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Molecular method for identification of Rickettsia species in clinical and environmental samples.

Jado Isabel I   Escudero Raquel R   Gil Horacio H   Jiménez-Alonso María Isabel MI   Sousa Rita R   García-Pérez Ana L AL   Rodríguez-Vargas Manuela M   Lobo Bruno B   Anda Pedro P  

Journal of clinical microbiology 20061011 12


We present a PCR method targeting the 23S-5S internal transcribed spacer coupled with reverse line blotting that allows Rickettsia species detection and identification in a single step. The method is highly sensitive and specific in identifying Rickettsia species from both patient and environmental samples. The generic approach used allowed us to identify new pathogens. ...[more]

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