Ontology highlight
ABSTRACT:
SUBMITTER: Raymond CS
PROVIDER: S-EPMC1764711 | biostudies-literature | 2007 Jan
REPOSITORIES: biostudies-literature
Raymond Christopher S CS Soriano Philippe P
PloS one 20070117 1
DNA site-specific recombinases (SSRs) such as Cre, FLPe, and phiC31, are powerful tools for analyzing gene function in vertebrates. While the availability of multiple high-efficiency SSRs would facilitate a wide array of genomic engineering possibilities, efficient recombination in mammalian cells has only been observed with Cre recombinase. Here we report the de novo synthesis of mouse codon-optimized FLP (FLPo) and PhiC31 (PhiC31o) SSRs, which result in recombination efficiencies similar to Cr ...[more]