Ontology highlight
ABSTRACT:
SUBMITTER: Thyagarajan B
PROVIDER: S-EPMC87055 | biostudies-literature | 2001 Jun
REPOSITORIES: biostudies-literature
Thyagarajan B B Olivares E C EC Hollis R P RP Ginsburg D S DS Calos M P MP
Molecular and cellular biology 20010601 12
We previously established that the phage phiC31 integrase, a site-specific recombinase, mediates efficient integration in the human cell environment at attB and attP phage attachment sites on extrachromosomal vectors. We show here that phage attP sites inserted at various locations in human and mouse chromosomes serve as efficient targets for precise site-specific integration. Moreover, we characterize native "pseudo" attP sites in the human and mouse genomes that also mediate efficient integras ...[more]