Ontology highlight
ABSTRACT:
SUBMITTER: Heegaard NH
PROVIDER: S-EPMC17817 | biostudies-literature | 2000
REPOSITORIES: biostudies-literature
Heegaard N H NH Larsen M R MR Muncrief T T Wiik A A Roepstorff P P
Arthritis research 20000711 5
The antigenic specificity of an unusual antinuclear antibody pattern in three patient sera was identified after separating HeLa-cell nuclear extracts by two-dimensional (2D) gel electrophoresis and localizing the antigens by immunoblotting with patient serum. Protein spots were excised from the 2D gel and their contents were analyzed by matrix-assisted laser desorption-ionization (MALDI) or nanoelectrospray ionization time-of-flight (TOF) tandem mass spectrometry (MS) after in-gel digestion with ...[more]