Dataset Information

The CD34-related molecule podocalyxin is a potent inducer of microvillus formation.

ABSTRACT:

Background

Podocalyxin is a CD34-related transmembrane protein involved in hematopoietic cell homing, kidney morphogenesis, breast cancer progression, and epithelial cell polarization. Although this sialomucin has been shown to block cell adhesion, the mechanisms involved remain enigmatic. It has, however, been postulated that the adaptor proteins NHERF-1 and 2 could regulate apical targeting of Podocalyxin by linking it to the actin cytoskeleton.

Principal findings

Here, in contrast, we find that full-length Podocalyxin acts to recruit NHERF-1 to the apical domain. Moreover, we show that ectopic expression of Podocalyxin in epithelial cells leads to microvillus formation along an expanded apical domain that extends laterally to the junctional complexes. Removal of the C-terminal PDZ-binding domain of Podocalyxin abolishes NHERF-1 recruitment but, surprisingly, has no effect on the formation of microvilli. Instead, we find that the extracellular domain and transmembrane region of Podocalyxin are sufficient to direct recruitment of filamentous actin and ezrin to the plasma membrane and induce microvillus formation.

Conclusions/significance

Our data suggest that this single molecule can modulate NHERF localization and, independently, act as a key orchestrator of apical cell morphology, thereby lending mechanistic insights into its multiple roles as a polarity regulator, tumor progression marker, and anti-adhesin.

SUBMITTER: Nielsen JS

PROVIDER: S-EPMC1796660 | biostudies-literature | 2007 Feb

REPOSITORIES: biostudies-literature

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Publications

The CD34-related molecule podocalyxin is a potent inducer of microvillus formation.

Nielsen Julie S JS Graves Marcia L ML Chelliah Shierley S Vogl A Wayne AW Roskelley Calvin D CD McNagny Kelly M KM

PloS one 20070221 2

<h4>Background</h4>Podocalyxin is a CD34-related transmembrane protein involved in hematopoietic cell homing, kidney morphogenesis, breast cancer progression, and epithelial cell polarization. Although this sialomucin has been shown to block cell adhesion, the mechanisms involved remain enigmatic. It has, however, been postulated that the adaptor proteins NHERF-1 and 2 could regulate apical targeting of Podocalyxin by linking it to the actin cytoskeleton.<h4>Principal findings</h4>Here, in contr ...[more]

PMID: 17311105

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Dataset Information

The CD34-related molecule podocalyxin is a potent inducer of microvillus formation.

Background

Principal findings

Conclusions/significance

Publications

The CD34-related molecule podocalyxin is a potent inducer of microvillus formation.

Similar Datasets

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