Single-nucleotide-polymorphism mapping of the Pseudomonas aeruginosa type III secretion toxins for development of a diagnostic multiplex PCR system.
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ABSTRACT:
SUBMITTER: Ajayi T
PROVIDER: S-EPMC179785 | biostudies-literature | 2003 Aug
REPOSITORIES: biostudies-literature
Publications
Single-nucleotide-polymorphism mapping of the Pseudomonas aeruginosa type III secretion toxins for development of a diagnostic multiplex PCR system.
Ajayi Temitayo T Allmond Leonard R LR Sawa Teiji T Wiener-Kronish Jeanine P JP
Journal of clinical microbiology 20030801 8
We mapped the coding single nucleotide polymorphisms in four toxin genes-exoS, exoT, exoU, and exoY-of the Pseudomonas aeruginosa type III secretion system among several clinical isolates. We then used this information to design a multiplex PCR assay based on the simultaneous amplification of fragments of these genes. Eight strains of known genotype were used to test our multiplex PCR method, which showed 100% sensitivity and specificity in this small sample size. This assay appears to be promis ...[more]
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