Ontology highlight
ABSTRACT:
SUBMITTER: Kipnis Y
PROVIDER: S-EPMC1805612 | biostudies-literature | 2007 Feb
REPOSITORIES: biostudies-literature
Kipnis Yakov Y Papo Niv N Haran Gilad G Horovitz Amnon A
Proceedings of the National Academy of Sciences of the United States of America 20070221 9
The double-ring chaperonin GroEL mediates protein folding, in conjunction with its helper protein GroES, by undergoing ATP-induced conformational changes that are concerted within each heptameric ring. Here we have examined whether the concerted nature of these transitions is responsible for protein substrate release in an all-or-none manner. Two chimeric substrates were designed, each with two different reporter activities that were recovered after denaturation in GroES-dependent and independen ...[more]