Unknown

Dataset Information

0

Crucial role of Bysl in mammalian preimplantation development as an integral factor for 40S ribosome biogenesis.


ABSTRACT: Blastocyst formation during mammalian preimplantation development is a unique developmental process that involves lineage segregation between the inner cell mass and the trophectoderm. To elucidate the molecular mechanisms underlying blastocyst formation, we have functionally screened a subset of preimplantation embryo-associated transcripts by using small interfering RNA (siRNA) and identified Bysl (bystin-like) as an essential gene for this process. The development of embryos injected with Bysl siRNA was arrested just prior to blastocyst formation, resulting in a defect in trophectoderm differentiation. Silencing of Bysl by using an episomal short hairpin RNA expression vector inhibited proliferation of embryonic stem cells. Exogenously expressed Bysl tagged with a fluorescent protein was concentrated in the nucleolus with a diffuse nucleoplasmic distribution. Furthermore, the loss of Bysl function by using RNA interference or dominant negative mutants caused defects in 40S ribosomal subunit biogenesis. These findings provide evidence for a crucial role of Bysl as an integral factor for ribosome biogenesis and suggest a critical dependence of blastocyst formation on active translation machinery.

SUBMITTER: Adachi K 

PROVIDER: S-EPMC1820511 | biostudies-literature | 2007 Mar

REPOSITORIES: biostudies-literature

altmetric image

Publications

Crucial role of Bysl in mammalian preimplantation development as an integral factor for 40S ribosome biogenesis.

Adachi Kenjiro K   Soeta-Saneyoshi Chie C   Sagara Hiroshi H   Iwakura Yoichiro Y  

Molecular and cellular biology 20070122 6


Blastocyst formation during mammalian preimplantation development is a unique developmental process that involves lineage segregation between the inner cell mass and the trophectoderm. To elucidate the molecular mechanisms underlying blastocyst formation, we have functionally screened a subset of preimplantation embryo-associated transcripts by using small interfering RNA (siRNA) and identified Bysl (bystin-like) as an essential gene for this process. The development of embryos injected with Bys  ...[more]

Similar Datasets

| S-EPMC3559688 | biostudies-literature
| S-EPMC4895721 | biostudies-literature
| S-EPMC1280274 | biostudies-literature
| S-EPMC7549620 | biostudies-literature
| S-EPMC3907300 | biostudies-literature
| S-EPMC3112407 | biostudies-literature
| S-EPMC60282 | biostudies-literature
| S-EPMC5416842 | biostudies-literature
| S-EPMC1360730 | biostudies-other
| S-EPMC3279398 | biostudies-literature