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Rapid molecular strategy for filovirus detection and characterization.


ABSTRACT: Filoviruses have the capacity to cause lethal outbreaks of hemorrhagic fever in primates. Here we present a simple consensus reverse transcription-PCR method for filovirus recognition and characterization and demonstrate its utility with all known filovirus strains. Phylogenetic assignment is achieved by automated web-based sequence analysis of amplification products.

SUBMITTER: Zhai J 

PROVIDER: S-EPMC1828965 | biostudies-literature | 2007 Jan

REPOSITORIES: biostudies-literature

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Filoviruses have the capacity to cause lethal outbreaks of hemorrhagic fever in primates. Here we present a simple consensus reverse transcription-PCR method for filovirus recognition and characterization and demonstrate its utility with all known filovirus strains. Phylogenetic assignment is achieved by automated web-based sequence analysis of amplification products. ...[more]

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