Rapid molecular strategy for orbivirus detection and characterization.
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ABSTRACT:
SUBMITTER: Palacios G
PROVIDER: S-EPMC3122741 | biostudies-literature | 2011 Jun
REPOSITORIES: biostudies-literature
Publications
Rapid molecular strategy for orbivirus detection and characterization.
Palacios Gustavo G Cowled Chris C Bussetti Ana V AV Savji Nazir N Weir Richard R Wick Ivan I Travassos da Rosa Amelia A Calisher Charles H CH Tesh Robert B RB Boyle David D Lipkin W Ian WI
Journal of clinical microbiology 20110330 6
Orbiviruses infect a wide range of hosts, including humans. The ability to detect them has been hampered by their diversity. Here we present a simple consensus reverse transcription (RT)-PCR method targeting the polymerase gene for orbivirus recognition and characterization. Phylogenetic assignment is achieved by automated Web-based sequence analysis of amplification products. ...[more]
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