PCR-based diagnosis of Naegleria sp. infection in formalin-fixed and paraffin-embedded brain sections.
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ABSTRACT:
SUBMITTER: Schild M
PROVIDER: S-EPMC1828997 | biostudies-literature | 2007 Feb
REPOSITORIES: biostudies-literature
Publications
PCR-based diagnosis of Naegleria sp. infection in formalin-fixed and paraffin-embedded brain sections.
Schild Marc M Gianinazzi Christian C Gottstein Bruno B Müller Norbert N
Journal of clinical microbiology 20061122 2
We developed a real-time PCR which allowed the highly sensitive detection of Naegleria fowleri in histological brain tissue sections from experimentally infected mice. This genus-specific small-subunit (18S) rRNA gene-based PCR can complement conventional (immuno-) histology for the diagnosis of primary amoebic meningoencephalitis in paraffin-embedded brain necropsy specimens that had been fixed in formalin buffered with phosphate-buffered saline. ...[more]
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