ACE2 chromogenic immunostaining protocol optimized for formalin-fixed paraffin-embedded human tissue sections.
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ABSTRACT:
SUBMITTER: Jorgensen M
PROVIDER: S-EPMC8292114 | biostudies-literature | 2021 Sep
REPOSITORIES: biostudies-literature
Publications
ACE2 chromogenic immunostaining protocol optimized for formalin-fixed paraffin-embedded human tissue sections.
Jorgensen Marda M Joseph Paul P Posgai Amanda L AL Vander Heide Richard S RS Kusmartseva Irina I Atkinson Mark A MA
STAR protocols 20210721 3
Angiotensin-converting enzyme 2 (ACE2) is a key cellular entry factor for severe acute respiratory syndrome coronavirus 2. Hence, identifying cell types that express ACE2 is important for understanding the pathophysiology of coronavirus disease 2019. We performed extensive testing of multiple primary antibodies across various human tissue types. Here, we describe an optimized protocol for immunostaining of ACE2 in formalin-fixed paraffin-embedded human pancreas, small intestine, and kidney tissu ...[more]
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