Ontology highlight
ABSTRACT:
SUBMITTER: Gorski JJ
PROVIDER: S-EPMC1829371 | biostudies-literature | 2007 Mar
REPOSITORIES: biostudies-literature
Gorski Julia J JJ Pathak Shalini S Panov Kostya K Kasciukovic Taciana T Panova Tanya T Russell Jackie J Zomerdijk Joost C B M JC
The EMBO journal 20070222 6
In mammalian RNA polymerase I transcription, SL1, an assembly of TBP and associated factors (TAFs), is essential for preinitiation complex formation at ribosomal RNA gene promoters in vitro. We provide evidence for a novel component of SL1, TAF(I)41 (MGC5306), which functions in Pol I transcription. TAF(I)41 resides at the rDNA promoter in the nucleolus and co-purifies and co-immunoprecipitates with SL1. TAF(I)41 immunodepletion from nuclear extracts dramatically reduces Pol I transcription; add ...[more]