Unknown

Dataset Information

0

Reconfiguration of yeast 40S ribosomal subunit domains by the translation initiation multifactor complex.


ABSTRACT: In the process of protein synthesis, the small (40S) subunit of the eukaryotic ribosome is recruited to the capped 5' end of the mRNA, from which point it scans along the 5' untranslated region in search of a start codon. However, the 40S subunit alone is not capable of functional association with cellular mRNA species; it has to be prepared for the recruitment and scanning steps by interactions with a group of eukaryotic initiation factors (eIFs). In budding yeast, an important subset of these factors (1, 2, 3, and 5) can form a multifactor complex (MFC). Here, we describe cryo-EM reconstructions of the 40S subunit, of the MFC, and of 40S complexes with MFC factors plus eIF1A. These studies reveal the positioning of the core MFC on the 40S subunit, and show how eIF-binding induces mobility in the head and platform and reconfigures the head-platform-body relationship. This is expected to increase the accessibility of the mRNA channel, thus enabling the 40S subunit to convert to a recruitment-competent state.

SUBMITTER: Gilbert RJ 

PROVIDER: S-EPMC1832216 | biostudies-literature | 2007 Apr

REPOSITORIES: biostudies-literature

altmetric image

Publications

Reconfiguration of yeast 40S ribosomal subunit domains by the translation initiation multifactor complex.

Gilbert Robert J C RJ   Gordiyenko Yulya Y   von der Haar Tobias T   Sonnen Andreas F-P AF   Hofmann Gregor G   Nardelli Maria M   Stuart David I DI   McCarthy John E G JE  

Proceedings of the National Academy of Sciences of the United States of America 20070326 14


In the process of protein synthesis, the small (40S) subunit of the eukaryotic ribosome is recruited to the capped 5' end of the mRNA, from which point it scans along the 5' untranslated region in search of a start codon. However, the 40S subunit alone is not capable of functional association with cellular mRNA species; it has to be prepared for the recruitment and scanning steps by interactions with a group of eukaryotic initiation factors (eIFs). In budding yeast, an important subset of these  ...[more]

Similar Datasets

| S-EPMC3258154 | biostudies-literature
| S-EPMC2731904 | biostudies-literature
| S-EPMC4151992 | biostudies-literature
| S-EPMC8997904 | biostudies-literature
| S-EPMC3390373 | biostudies-literature
| S-EPMC3543093 | biostudies-literature
| S-EPMC6298780 | biostudies-literature
| S-EPMC3394201 | biostudies-literature
| S-EPMC10710265 | biostudies-literature
| S-EPMC6614841 | biostudies-literature