Unknown

Dataset Information

0

Translational initiation factor eIF5 replaces eIF1 on the 40S ribosomal subunit to promote start-codon recognition.


ABSTRACT: In eukaryotic translation initiation, AUG recognition of the mRNA requires accommodation of Met-tRNAi in a 'PIN' state, which is antagonized by the factor eIF1. eIF5 is a GTPase activating protein (GAP) of eIF2 that additionally promotes stringent AUG selection, but the molecular basis of its dual function was unknown. We present a cryo-electron microscopy (cryo-EM) reconstruction of a yeast 48S pre-initiation complex (PIC), at an overall resolution of 3.0 Å, featuring the N-terminal domain (NTD) of eIF5 bound to the 40S subunit at the location vacated by eIF1. eIF5 interacts with and allows a more accommodated orientation of Met-tRNAi. Substitutions of eIF5 residues involved in the eIF5-NTD/tRNAi interaction influenced initiation at near-cognate UUG codonsin vivo, and the closed/open PIC conformation in vitro, consistent with direct stabilization of the codon:anticodon duplex by the wild-type eIF5-NTD. The present structure reveals the basis for a key role of eIF5 in start-codon selection.

SUBMITTER: Llacer JL 

PROVIDER: S-EPMC6298780 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

altmetric image

Publications

Translational initiation factor eIF5 replaces eIF1 on the 40S ribosomal subunit to promote start-codon recognition.

Llácer Jose Luis JL   Hussain Tanweer T   Saini Adesh K AK   Nanda Jagpreet Singh JS   Nanda Jagpreet Singh JS   Kaur Sukhvir S   Gordiyenko Yuliya Y   Kumar Rakesh R   Hinnebusch Alan G AG   Lorsch Jon R JR   Ramakrishnan V V  

eLife 20181130


In eukaryotic translation initiation, AUG recognition of the mRNA requires accommodation of Met-tRNA<sub>i</sub> in a 'P<sub>IN</sub>' state, which is antagonized by the factor eIF1. eIF5 is a GTPase activating protein (GAP) of eIF2 that additionally promotes stringent AUG selection, but the molecular basis of its dual function was unknown. We present a cryo-electron microscopy (cryo-EM) reconstruction of a yeast 48S pre-initiation complex (PIC), at an overall resolution of 3.0 Å, featuring the  ...[more]

Similar Datasets

| S-EPMC1865493 | biostudies-literature
| S-EPMC6735954 | biostudies-literature
| S-EPMC2783965 | biostudies-literature
| S-EPMC3326321 | biostudies-literature
| S-EPMC4151992 | biostudies-literature
| S-EPMC1832216 | biostudies-literature
| S-EPMC3917153 | biostudies-literature
| S-EPMC3258154 | biostudies-literature
| S-EPMC2998303 | biostudies-literature
2023-12-02 | GSE230189 | GEO