Ontology highlight
ABSTRACT:
SUBMITTER: Llacer JL
PROVIDER: S-EPMC6298780 | biostudies-literature | 2018 Nov
REPOSITORIES: biostudies-literature
Llácer Jose Luis JL Hussain Tanweer T Saini Adesh K AK Nanda Jagpreet Singh JS Nanda Jagpreet Singh JS Kaur Sukhvir S Gordiyenko Yuliya Y Kumar Rakesh R Hinnebusch Alan G AG Lorsch Jon R JR Ramakrishnan V V
eLife 20181130
In eukaryotic translation initiation, AUG recognition of the mRNA requires accommodation of Met-tRNA<sub>i</sub> in a 'P<sub>IN</sub>' state, which is antagonized by the factor eIF1. eIF5 is a GTPase activating protein (GAP) of eIF2 that additionally promotes stringent AUG selection, but the molecular basis of its dual function was unknown. We present a cryo-electron microscopy (cryo-EM) reconstruction of a yeast 48S pre-initiation complex (PIC), at an overall resolution of 3.0 Å, featuring the ...[more]