Unknown

Dataset Information

0

A TOM1 homologue is required for multiplication of Tobacco mosaic virus in Nicotiana benthamiana.

ABSTRACT: The AtTOM1 gene of Arabidopsis thaliana had been shown to be essential for the efficient multiplication of Tobacco mosaic virus (TMV) in A. thaliana. In this study, we cloned an AtTOM1-like gene from Nicotiana benthamiana named as NbTOM1. Sequence alignment showed that NbTOM1 is closely related to AtTOM1 homologues of N. tabacum and Lycopersicon esculentum with 97.2% and 92.6% nucleotide sequence identities, respectively. Silencing of NbTOM1 by a modified viral satellite DNA-based vector resulted in complete inhibition of the multiplication of TMV in N. benthamiana. The result suggests that inhibition of NbTOM1 via RNA silencing is a potentially useful method for generating TMV-resistant plants.

SUBMITTER: Chen B 

PROVIDER: S-EPMC1838827 | biostudies-literature | 2007 Apr

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

A TOM1 homologue is required for multiplication of Tobacco mosaic virus in Nicotiana benthamiana.

Chen Bing B   Jiang Jin-hua JH   Zhou Xue-ping XP  

Journal of Zhejiang University. Science. B 20070401 4

The AtTOM1 gene of Arabidopsis thaliana had been shown to be essential for the efficient multiplication of Tobacco mosaic virus (TMV) in A. thaliana. In this study, we cloned an AtTOM1-like gene from Nicotiana benthamiana named as NbTOM1. Sequence alignment showed that NbTOM1 is closely related to AtTOM1 homologues of N. tabacum and Lycopersicon esculentum with 97.2% and 92.6% nucleotide sequence identities, respectively. Silencing of NbTOM1 by a modified viral satellite DNA-based vector resulte  ...[more]

Similar Datasets

Transcriptomics Tobacco mosaic virus infection alters phloem associated translatomes in Nicotiana benthamiana
2017-07-17 | GSE97663 | GEO
Unknown A Conserved Carboxylesterase Inhibits Tobacco mosaic virus (TMV) Accumulation in Nicotiana benthamiana Plants.
| S-EPMC7077250 | biostudies-literature
Unknown Role of brassinosteroid signaling in modulating Tobacco mosaic virus resistance in Nicotiana benthamiana.
| S-EPMC4738339 | biostudies-literature
Unknown Ethylene-induced NbMYB4L is involved in resistance against tobacco mosaic virus in Nicotiana benthamiana.
| S-EPMC8659562 | biostudies-literature
Unknown Sugarcane Mosaic Virus-Based Gene Silencing in Nicotiana benthamiana.
| S-EPMC5903913 | biostudies-literature
Unknown Alpha-momorcharin enhances Nicotiana benthamiana resistance to tobacco mosaic virus infection through modulation of reactive oxygen species.
| S-EPMC7411664 | biostudies-literature
Unknown Identification of the Virulence Factors of Candidatus Liberibacter asiaticus via Heterologous Expression in Nicotiana benthamiana using Tobacco Mosaic Virus.
| S-EPMC6888081 | biostudies-literature
Unknown Transcriptome analysis of Nicotiana benthamiana infected by Tobacco curly shoot virus.
| S-EPMC6122796 | biostudies-literature
Unknown Proteomic and Phosphoproteomic Analysis in Tobacco Mosaic Virus-Infected Tobacco (Nicotiana tabacum).
| S-EPMC6406717 | biostudies-literature
Unknown Genome-Wide Characterization of miRNAs Involved in N Gene-Mediated Immunity in Response to Tobacco Mosaic Virus in Nicotiana benthamiana.
| S-EPMC4294599 | biostudies-literature