Detection of low-copy-number genomic DNA sequences in individual bacterial cells by using peptide nucleic acid-assisted rolling-circle amplification and fluorescence in situ hybridization.
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ABSTRACT:
SUBMITTER: Smolina I
PROVIDER: S-EPMC1855634 | biostudies-literature | 2007 Apr
REPOSITORIES: biostudies-literature
Publications
Detection of low-copy-number genomic DNA sequences in individual bacterial cells by using peptide nucleic acid-assisted rolling-circle amplification and fluorescence in situ hybridization.
Smolina Irina I Lee Charles C Frank-Kamenetskii Maxim M
Applied and environmental microbiology 20070209 7
An approach is proposed for in situ detection of short signature DNA sequences present in single copies per bacterial genome. The site is locally opened by peptide nucleic acids, and a circular oligonucleotide is assembled. The amplicon generated by rolling circle amplification is detected by hybridization with fluorescently labeled decorator probes. ...[more]
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