Ontology highlight
ABSTRACT:
SUBMITTER: Beals TP
PROVIDER: S-EPMC3017024 | biostudies-literature | 2010
REPOSITORIES: biostudies-literature
Beals Thomas P TP Smith James H JH Nietupski Raymond M RM Lane David J DJ
BMC molecular biology 20101207
<h4>Background</h4>Amplification of single-stranded DNA circles has wide utility for a variety of applications. The two-primer ramified rolling circle amplification (RAM) reaction provides exponential DNA amplification under isothermal conditions, creating a regular laddered series of double-stranded DNA products. However, the molecular mechanism of the RAM reaction remains unexplained.<h4>Results</h4>A RAM reaction model predicts exponential accumulation of a double-stranded DNA product size se ...[more]