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Quantitative analysis of mouse urine volatiles: in search of MHC-dependent differences.

ABSTRACT: Genes of the major histocompatibility complex (MHC), which play a critical role in immune recognition, influence mating preference and other social behaviors in mice. Training experiments using urine scent from mice differing only in the MHC complex, from MHC class I mutants or from knock-out mice lacking functional MHC class I molecules (beta2m-deficient), suggest that these behavioral effects are mediated by differences in MHC-dependent volatile components. In search for the physical basis of these behavioral studies, we have conducted a comparison of urinary volatiles in three sub-strains of C57BL/6 mice, a beta2m-deficient mutant lacking functional MHC class I expression and two unrelated inbred strains, using the technique of sorptive extraction with polydimethylsiloxan and subsequent analysis by gas chromatography/mass spectrometry. We show (i) that qualitative differences occur between different inbred strains but not in mice with the C57BL/6 background, (ii) that the individual variability in abundance in the same mouse strain is strongly component-dependent, (iii) that C57BL/6 sub-strains obtained from different provenance show a higher fraction of quantitative differences than a sub-strain and its beta2m-mutant obtained from the same source and (iv) that comparison of the spectra of beta2m mice and the corresponding wild type reveals no qualitative differences in close to 200 major and minor components and only minimal differences in a few substances from an ensemble of 69 selected for quantitative analysis. Our data suggest that odor is shaped by ontogenetic, environmental and genetic factors, and the gestalt of this scent may identify a mouse on the individual and population level; but, within the limits of the ensemble of components analysed, the results do not support the notion that functional MHC class I molecules influence the urinary volatile composition.

SUBMITTER: Rock F

PROVIDER: S-EPMC1855987 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:Simple Summary Biodiversity conservation is one of the most relevant current issues. The safeguarding of genetic resources of autochthon farm animals at risk of extinction, such as the Teramana goat, reflects the enhancement of the relative animal productions, in order to highlight the peculiarities that can induce breeders to devote themselves to a specific type of breeding and that can attract the interests of potential consumers. Recent advances in proteomics and bioinformatics tools offer the potential to investigate breed differences at the level of proteins (proteome) and consequently in terms of the biological processes and molecular functions of the specific breed under study. However, there is a lack of information on the potentiality of autochthonous breeds such as the Teramana goat. Therefore, in this study, a global differential proteomic experiment was carried out with the purpose of identifying variations in the expression of muscle proteins between the autochthon Teramana goat and the breed commonly used by the industry, Saanen. The results showed a difference in the expression pattern of 41 proteins that clearly separate the two breeds (underlying genetic differences), highlighting biological features that could influence the productivity, resilience and other traits of these animals. Abstract The intensification and standardization of livestock farming are causing a decline in the number of animal breeds in many species, such as the goat. The availability of more studies on the potentiality of goat breeds could raise awareness of their importance, conservation and productive possibilities. Label-free quantitative analysis was applied in this study to investigate the proteomic differences between the autochthon Teramana and Saanen goats that could be useful for defining peculiar features of these breeds. A total of 2093 proteins were characterized in the muscle exudate proteome of the Teramana and Saanen breeds. A total of 41 proteins clearly separated the two breeds. Eukaryotic initiation factor proteins and aldehyde-dehydrogenase 7 family-member A1 were up-regulated in the autochthon breed and associated with its resilience, whereas catalase was down-regulated and associated with lower muscular mass. This study is the most detailed report of goat muscle proteome. Several differentially regulated proteins between the two breeds were identified, providing insights into functional pathways that define this organism and its biology.

Project description:BackgroundAlthough autism is often characterized in literature by the presence of repetitive behavior, in structured decision tasks, individuals with autism spectrum disorder (ASD) have been found to examine more options in a given time period than controls.ObjectiveWe aimed to examine whether this investigative tendency emerges in information searches conducted via the internet.MethodsIn total, 1746 search engine users stated that they had ASD in 2019. This group's naturally occurring responses following 1491 unique general queries and 78 image queries were compared to those of all other users of the search engine. The main dependent measure was scrolled distance, which denoted the extent to which additional results were scanned beyond the initial results presented on-screen. Additionally, we examined the number of clicks on search results as an indicator of the degree of search outcome exploitation and assessed whether there was a trade-off between increased search range and the time invested in viewing initial search results.ResultsAfter issuing general queries, individuals with self-stated ASD scanned more results than controls. The scrolled distance in the results page of general queries was 45% larger for the group of individuals with ASD (P<.001; d=0.45). The group of individuals with ASD also made the first scroll faster than the controls (P<.001; d=0.51). The differences in scrolled distance were larger for popular queries. No group differences in scrolled distance emerged for image queries, suggesting that visual load impeded the investigative behavior of individuals with ASD. No differences emerged in the number of clicks on search results.ConclusionsIndividuals who self-stated that they had ASD scrutinized more general search results and fewer image search results than the controls. Thus, our results at least partially support the notion that individuals with ASD exhibit investigative behaviors and suggest that textual searches are an important context for expressing such tendencies.

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Quantitative analysis of mouse urine volatiles: in search of MHC-dependent differences.

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