Ontology highlight
ABSTRACT:
SUBMITTER: Fiala KA
PROVIDER: S-EPMC1866274 | biostudies-literature | 2007 Jan
REPOSITORIES: biostudies-literature
Fiala Kevin A KA Brown Jessica A JA Ling Hong H Kshetry Ajay K AK Zhang Jun J Taylor John-Stephen JS Yang Wei W Suo Zucai Z
Journal of molecular biology 20061007 3
Numerous template-dependent DNA polymerases are capable of catalyzing template-independent nucleotide additions onto blunt-end DNA. Such non-canonical activity has been hypothesized to increase the genomic hypermutability of retroviruses including human immunodeficiency viruses. Here, we employed pre-steady state kinetics and X-ray crystallography to establish a mechanism for blunt-end additions catalyzed by Sulfolobus solfataricus Dpo4. Our kinetic studies indicated that the first blunt-end dAT ...[more]