Transient-State Kinetic Analysis of the RNA Polymerase I Nucleotide Incorporation Mechanism.

ABSTRACT: Eukaryotes express three or more multisubunit nuclear RNA polymerases (Pols) referred to as Pols I, II, and III, each of which synthesizes a specific subset of RNAs. Consistent with the diversity of their target genes, eukaryotic cells have evolved divergent cohorts of transcription factors and enzymatic properties for each RNA polymerase system. Over the years, many trans-acting factors that orchestrate transcription by the individual Pols have been described; however, little effort has been devoted to characterizing the molecular mechanisms of Pol I activity. To begin to address this gap in our understanding of eukaryotic gene expression, here we establish transient-state kinetic approaches to characterize the nucleotide incorporation mechanism of Pol I. We collected time courses for sin

SUBMITTER: Appling FD 

PROVIDER: S-EPMC4675888 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature