Ontology highlight
ABSTRACT:
SUBMITTER: Sefcikova J
PROVIDER: S-EPMC1874588 | biostudies-literature | 2007
REPOSITORIES: biostudies-literature
Sefcikova Jana J Krasovska Maryna V MV Sponer Jirí J Walter Nils G NG
Nucleic acids research 20070302 6
The genome of the human hepatitis delta virus (HDV) harbors a self-cleaving catalytic RNA motif, the genomic HDV ribozyme, whose crystal structure shows the dangling nucleotides 5' of the cleavage site projecting away from the catalytic core. This 5'-sequence contains a clinically conserved U-1 that we find to be essential for fast cleavage, as the order of activity follows U-1 > C-1 > A-1 > G-1, with a >25-fold activity loss from U-1 to G-1. Terbium(III) footprinting detects conformations for t ...[more]