Ontology highlight
ABSTRACT:
SUBMITTER: Volna P
PROVIDER: S-EPMC1885675 | biostudies-literature | 2007
REPOSITORIES: biostudies-literature
Volná Petra P Jarjour Jordan J Baxter Sarah S Roffler Steve R SR Monnat Raymond J RJ Stoddard Barry L BL Scharenberg Andrew M AM
Nucleic acids research 20070410 8
LAGLIDADG homing endonucleases (LHEs) cleave 18-24 bp DNA sequences and are promising enzymes for applications requiring sequence-specific DNA cleavage amongst genome-sized DNA backgrounds. Here, we report a method for cell surface display of LHEs, which facilitates analysis of their DNA binding and cleavage properties by flow cytometry. Cells expressing surface LHEs can be stained with fluorescently conjugated double-stranded oligonucleotides (dsOligos) containing their respective target sequen ...[more]