Ontology highlight
ABSTRACT:
SUBMITTER: Arhel NJ
PROVIDER: S-EPMC1894778 | biostudies-literature | 2007 Jun
REPOSITORIES: biostudies-literature
Arhel Nathalie J NJ Souquere-Besse Sylvie S Munier Sandie S Souque Philippe P Guadagnini Stéphanie S Rutherford Sandra S Prévost Marie-Christine MC Allen Terry D TD Charneau Pierre P
The EMBO journal 20070607 12
The HIV-1 central DNA Flap acts as a cis-acting determinant of HIV-1 genome nuclear import. Indeed, DNA-Flap re-insertion within lentiviral-derived gene transfer vectors strongly stimulates gene transfer efficiencies. In this study, we sought to understand the mechanisms by which the central DNA Flap mediates HIV-1 nuclear import. Here, we show that reverse transcription (RT degrees) occurs within an intact capsid (CA) shell, independently of the routing process towards the nuclear membrane, and ...[more]