A major zebrafish polymorphism resource for genetic mapping.
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ABSTRACT:
SUBMITTER: Bradley KM
PROVIDER: S-EPMC1896001 | biostudies-literature | 2007
REPOSITORIES: biostudies-literature
Publications
A major zebrafish polymorphism resource for genetic mapping.
Bradley Kevin M KM Elmore J Bradford JB Breyer Joan P JP Yaspan Brian L BL Jessen Jason R JR Knapik Ela W EW Smith Jeffrey R JR
Genome biology 20070101 4
We have identified 645,088 candidate polymorphisms in zebrafish and observe a single nucleotide polymorphism (SNP) validation rate of 71% to 86%, improving with polymorphism confidence score. Variant sites are non-random, with an excess of specific novel T- and A-rich motifs. We positioned half of the polymorphisms on zebrafish genetic and physical maps as a resource for positional cloning. We further demonstrate bulked segregant analysis using the anchored SNPs as a method for high-throughput g ...[more]
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